cell fractionation kit Search Results


cell fractionation kit invent biotechnologies cat  (Invent Biotechnologies)
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Invent Biotechnologies cell fractionation kit invent biotechnologies cat
Cell Fractionation Kit Invent Biotechnologies Cat, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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carboxyphenol ba  (Chem Impex International)
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Chem Impex International carboxyphenol ba
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plasma membrane protein isolation kit  (Invent Biotechnologies)
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Invent Biotechnologies plasma membrane protein isolation kit
Plasma Membrane Protein Isolation Kit, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytoskeletal fractions  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc cytoskeletal fractions
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vimentin  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc vimentin
FIGURE 3 Ephrin-B2 ICD translocates to nucleus. (a) Schematic of ephrin-B2 shedding upon EphB1 receptor binding and cleavage by g-secretase. Upon binding of extracellular EphB1 receptor to ephrin-B2 (1), the ectodomain of ephrin B2 (B2 NTF) is shedded by ADAM activity (2), thereby also producing the membrane-tethered ephrin-B2 CTF. Subsequently, g-secretase cleaves the ephrin-B2 CTF in its transmembrane region (TMR) to produce the ephrin-B2 ICD (3). (b) Schematic of ephrin-B2 FL, CTF, and ICD constructs used for overex- pression in ESdM. The ephrin-B2 ICD contains several Src homology 2 (SH2) binding domains and one PDZ binding domain. (c) Western immunoblot of cell fractions: Membrane (marker: AIF), cytosol (marker: MEK1/2), and the nuclear fraction (marker: <t>vimentin).</t> This blot is representative for <t>the</t> <t>fractionation</t> control of all three samples shown in (d). M, membrane; C, cytosol; N, nucleus. (d) Cell fractionation of ESdM overexpressing the ephrin-B2 FL, CTF, or ICD constructs. The ephrin-B2 FL and CTF are detected in the membrane fraction. The ephrin-B2 ICD is detected in the cytosolic and nuclear fractions, indicating translocation of this fragment from the cytosol to the nucleus. Cell treatment with MG132 (0.1 mM, 4 hr) strongly stabilized the ephrin-B2 ICD
Vimentin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qproteome cell fractionation kit  (Qiagen)
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Qiagen qproteome cell fractionation kit
FIGURE 3 Ephrin-B2 ICD translocates to nucleus. (a) Schematic of ephrin-B2 shedding upon EphB1 receptor binding and cleavage by g-secretase. Upon binding of extracellular EphB1 receptor to ephrin-B2 (1), the ectodomain of ephrin B2 (B2 NTF) is shedded by ADAM activity (2), thereby also producing the membrane-tethered ephrin-B2 CTF. Subsequently, g-secretase cleaves the ephrin-B2 CTF in its transmembrane region (TMR) to produce the ephrin-B2 ICD (3). (b) Schematic of ephrin-B2 FL, CTF, and ICD constructs used for overex- pression in ESdM. The ephrin-B2 ICD contains several Src homology 2 (SH2) binding domains and one PDZ binding domain. (c) Western immunoblot of cell fractions: Membrane (marker: AIF), cytosol (marker: MEK1/2), and the nuclear fraction (marker: <t>vimentin).</t> This blot is representative for <t>the</t> <t>fractionation</t> control of all three samples shown in (d). M, membrane; C, cytosol; N, nucleus. (d) Cell fractionation of ESdM overexpressing the ephrin-B2 FL, CTF, or ICD constructs. The ephrin-B2 FL and CTF are detected in the membrane fraction. The ephrin-B2 ICD is detected in the cytosolic and nuclear fractions, indicating translocation of this fragment from the cytosol to the nucleus. Cell treatment with MG132 (0.1 mM, 4 hr) strongly stabilized the ephrin-B2 ICD
Qproteome Cell Fractionation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear/cytosolic fractionation kit  (Cell Biolabs Inc)
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Cell Biolabs Inc nuclear/cytosolic fractionation kit
FIGURE 3 Ephrin-B2 ICD translocates to nucleus. (a) Schematic of ephrin-B2 shedding upon EphB1 receptor binding and cleavage by g-secretase. Upon binding of extracellular EphB1 receptor to ephrin-B2 (1), the ectodomain of ephrin B2 (B2 NTF) is shedded by ADAM activity (2), thereby also producing the membrane-tethered ephrin-B2 CTF. Subsequently, g-secretase cleaves the ephrin-B2 CTF in its transmembrane region (TMR) to produce the ephrin-B2 ICD (3). (b) Schematic of ephrin-B2 FL, CTF, and ICD constructs used for overex- pression in ESdM. The ephrin-B2 ICD contains several Src homology 2 (SH2) binding domains and one PDZ binding domain. (c) Western immunoblot of cell fractions: Membrane (marker: AIF), cytosol (marker: MEK1/2), and the nuclear fraction (marker: <t>vimentin).</t> This blot is representative for <t>the</t> <t>fractionation</t> control of all three samples shown in (d). M, membrane; C, cytosol; N, nucleus. (d) Cell fractionation of ESdM overexpressing the ephrin-B2 FL, CTF, or ICD constructs. The ephrin-B2 FL and CTF are detected in the membrane fraction. The ephrin-B2 ICD is detected in the cytosolic and nuclear fractions, indicating translocation of this fragment from the cytosol to the nucleus. Cell treatment with MG132 (0.1 mM, 4 hr) strongly stabilized the ephrin-B2 ICD
Nuclear/Cytosolic Fractionation Kit, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoalert cell fractionation kit  (Becton Dickinson)
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Becton Dickinson apoalert cell fractionation kit
FIGURE 3 Ephrin-B2 ICD translocates to nucleus. (a) Schematic of ephrin-B2 shedding upon EphB1 receptor binding and cleavage by g-secretase. Upon binding of extracellular EphB1 receptor to ephrin-B2 (1), the ectodomain of ephrin B2 (B2 NTF) is shedded by ADAM activity (2), thereby also producing the membrane-tethered ephrin-B2 CTF. Subsequently, g-secretase cleaves the ephrin-B2 CTF in its transmembrane region (TMR) to produce the ephrin-B2 ICD (3). (b) Schematic of ephrin-B2 FL, CTF, and ICD constructs used for overex- pression in ESdM. The ephrin-B2 ICD contains several Src homology 2 (SH2) binding domains and one PDZ binding domain. (c) Western immunoblot of cell fractions: Membrane (marker: AIF), cytosol (marker: MEK1/2), and the nuclear fraction (marker: <t>vimentin).</t> This blot is representative for <t>the</t> <t>fractionation</t> control of all three samples shown in (d). M, membrane; C, cytosol; N, nucleus. (d) Cell fractionation of ESdM overexpressing the ephrin-B2 FL, CTF, or ICD constructs. The ephrin-B2 FL and CTF are detected in the membrane fraction. The ephrin-B2 ICD is detected in the cytosolic and nuclear fractions, indicating translocation of this fragment from the cytosol to the nucleus. Cell treatment with MG132 (0.1 mM, 4 hr) strongly stabilized the ephrin-B2 ICD
Apoalert Cell Fractionation Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell fractionation kit-standard  (MitoSciences)
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MitoSciences cell fractionation kit-standard
FIGURE 3 Ephrin-B2 ICD translocates to nucleus. (a) Schematic of ephrin-B2 shedding upon EphB1 receptor binding and cleavage by g-secretase. Upon binding of extracellular EphB1 receptor to ephrin-B2 (1), the ectodomain of ephrin B2 (B2 NTF) is shedded by ADAM activity (2), thereby also producing the membrane-tethered ephrin-B2 CTF. Subsequently, g-secretase cleaves the ephrin-B2 CTF in its transmembrane region (TMR) to produce the ephrin-B2 ICD (3). (b) Schematic of ephrin-B2 FL, CTF, and ICD constructs used for overex- pression in ESdM. The ephrin-B2 ICD contains several Src homology 2 (SH2) binding domains and one PDZ binding domain. (c) Western immunoblot of cell fractions: Membrane (marker: AIF), cytosol (marker: MEK1/2), and the nuclear fraction (marker: <t>vimentin).</t> This blot is representative for <t>the</t> <t>fractionation</t> control of all three samples shown in (d). M, membrane; C, cytosol; N, nucleus. (d) Cell fractionation of ESdM overexpressing the ephrin-B2 FL, CTF, or ICD constructs. The ephrin-B2 FL and CTF are detected in the membrane fraction. The ephrin-B2 ICD is detected in the cytosolic and nuclear fractions, indicating translocation of this fragment from the cytosol to the nucleus. Cell treatment with MG132 (0.1 mM, 4 hr) strongly stabilized the ephrin-B2 ICD
Cell Fractionation Kit Standard, supplied by MitoSciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoalerts cell fractionation kit  (Becton Dickinson)
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Becton Dickinson apoalerts cell fractionation kit
FIGURE 3 Ephrin-B2 ICD translocates to nucleus. (a) Schematic of ephrin-B2 shedding upon EphB1 receptor binding and cleavage by g-secretase. Upon binding of extracellular EphB1 receptor to ephrin-B2 (1), the ectodomain of ephrin B2 (B2 NTF) is shedded by ADAM activity (2), thereby also producing the membrane-tethered ephrin-B2 CTF. Subsequently, g-secretase cleaves the ephrin-B2 CTF in its transmembrane region (TMR) to produce the ephrin-B2 ICD (3). (b) Schematic of ephrin-B2 FL, CTF, and ICD constructs used for overex- pression in ESdM. The ephrin-B2 ICD contains several Src homology 2 (SH2) binding domains and one PDZ binding domain. (c) Western immunoblot of cell fractions: Membrane (marker: AIF), cytosol (marker: MEK1/2), and the nuclear fraction (marker: <t>vimentin).</t> This blot is representative for <t>the</t> <t>fractionation</t> control of all three samples shown in (d). M, membrane; C, cytosol; N, nucleus. (d) Cell fractionation of ESdM overexpressing the ephrin-B2 FL, CTF, or ICD constructs. The ephrin-B2 FL and CTF are detected in the membrane fraction. The ephrin-B2 ICD is detected in the cytosolic and nuclear fractions, indicating translocation of this fragment from the cytosol to the nucleus. Cell treatment with MG132 (0.1 mM, 4 hr) strongly stabilized the ephrin-B2 ICD
Apoalerts Cell Fractionation Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ms861 cell fractionation kit  (MitoSciences)
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MitoSciences ms861 cell fractionation kit
FIGURE 3 Ephrin-B2 ICD translocates to nucleus. (a) Schematic of ephrin-B2 shedding upon EphB1 receptor binding and cleavage by g-secretase. Upon binding of extracellular EphB1 receptor to ephrin-B2 (1), the ectodomain of ephrin B2 (B2 NTF) is shedded by ADAM activity (2), thereby also producing the membrane-tethered ephrin-B2 CTF. Subsequently, g-secretase cleaves the ephrin-B2 CTF in its transmembrane region (TMR) to produce the ephrin-B2 ICD (3). (b) Schematic of ephrin-B2 FL, CTF, and ICD constructs used for overex- pression in ESdM. The ephrin-B2 ICD contains several Src homology 2 (SH2) binding domains and one PDZ binding domain. (c) Western immunoblot of cell fractions: Membrane (marker: AIF), cytosol (marker: MEK1/2), and the nuclear fraction (marker: <t>vimentin).</t> This blot is representative for <t>the</t> <t>fractionation</t> control of all three samples shown in (d). M, membrane; C, cytosol; N, nucleus. (d) Cell fractionation of ESdM overexpressing the ephrin-B2 FL, CTF, or ICD constructs. The ephrin-B2 FL and CTF are detected in the membrane fraction. The ephrin-B2 ICD is detected in the cytosolic and nuclear fractions, indicating translocation of this fragment from the cytosol to the nucleus. Cell treatment with MG132 (0.1 mM, 4 hr) strongly stabilized the ephrin-B2 ICD
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minute tm plasma membrane protein isolation and cell fractionation kit  (ScienCell)
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ScienCell minute tm plasma membrane protein isolation and cell fractionation kit
FIGURE 3 Ephrin-B2 ICD translocates to nucleus. (a) Schematic of ephrin-B2 shedding upon EphB1 receptor binding and cleavage by g-secretase. Upon binding of extracellular EphB1 receptor to ephrin-B2 (1), the ectodomain of ephrin B2 (B2 NTF) is shedded by ADAM activity (2), thereby also producing the membrane-tethered ephrin-B2 CTF. Subsequently, g-secretase cleaves the ephrin-B2 CTF in its transmembrane region (TMR) to produce the ephrin-B2 ICD (3). (b) Schematic of ephrin-B2 FL, CTF, and ICD constructs used for overex- pression in ESdM. The ephrin-B2 ICD contains several Src homology 2 (SH2) binding domains and one PDZ binding domain. (c) Western immunoblot of cell fractions: Membrane (marker: AIF), cytosol (marker: MEK1/2), and the nuclear fraction (marker: <t>vimentin).</t> This blot is representative for <t>the</t> <t>fractionation</t> control of all three samples shown in (d). M, membrane; C, cytosol; N, nucleus. (d) Cell fractionation of ESdM overexpressing the ephrin-B2 FL, CTF, or ICD constructs. The ephrin-B2 FL and CTF are detected in the membrane fraction. The ephrin-B2 ICD is detected in the cytosolic and nuclear fractions, indicating translocation of this fragment from the cytosol to the nucleus. Cell treatment with MG132 (0.1 mM, 4 hr) strongly stabilized the ephrin-B2 ICD
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Image Search Results


FIGURE 3 Ephrin-B2 ICD translocates to nucleus. (a) Schematic of ephrin-B2 shedding upon EphB1 receptor binding and cleavage by g-secretase. Upon binding of extracellular EphB1 receptor to ephrin-B2 (1), the ectodomain of ephrin B2 (B2 NTF) is shedded by ADAM activity (2), thereby also producing the membrane-tethered ephrin-B2 CTF. Subsequently, g-secretase cleaves the ephrin-B2 CTF in its transmembrane region (TMR) to produce the ephrin-B2 ICD (3). (b) Schematic of ephrin-B2 FL, CTF, and ICD constructs used for overex- pression in ESdM. The ephrin-B2 ICD contains several Src homology 2 (SH2) binding domains and one PDZ binding domain. (c) Western immunoblot of cell fractions: Membrane (marker: AIF), cytosol (marker: MEK1/2), and the nuclear fraction (marker: vimentin). This blot is representative for the fractionation control of all three samples shown in (d). M, membrane; C, cytosol; N, nucleus. (d) Cell fractionation of ESdM overexpressing the ephrin-B2 FL, CTF, or ICD constructs. The ephrin-B2 FL and CTF are detected in the membrane fraction. The ephrin-B2 ICD is detected in the cytosolic and nuclear fractions, indicating translocation of this fragment from the cytosol to the nucleus. Cell treatment with MG132 (0.1 mM, 4 hr) strongly stabilized the ephrin-B2 ICD

Journal: Glia

Article Title: Intramembranous processing by γ-secretase regulates reverse signaling of ephrin-B2 in migration of microglia.

doi: 10.1002/glia.23147

Figure Lengend Snippet: FIGURE 3 Ephrin-B2 ICD translocates to nucleus. (a) Schematic of ephrin-B2 shedding upon EphB1 receptor binding and cleavage by g-secretase. Upon binding of extracellular EphB1 receptor to ephrin-B2 (1), the ectodomain of ephrin B2 (B2 NTF) is shedded by ADAM activity (2), thereby also producing the membrane-tethered ephrin-B2 CTF. Subsequently, g-secretase cleaves the ephrin-B2 CTF in its transmembrane region (TMR) to produce the ephrin-B2 ICD (3). (b) Schematic of ephrin-B2 FL, CTF, and ICD constructs used for overex- pression in ESdM. The ephrin-B2 ICD contains several Src homology 2 (SH2) binding domains and one PDZ binding domain. (c) Western immunoblot of cell fractions: Membrane (marker: AIF), cytosol (marker: MEK1/2), and the nuclear fraction (marker: vimentin). This blot is representative for the fractionation control of all three samples shown in (d). M, membrane; C, cytosol; N, nucleus. (d) Cell fractionation of ESdM overexpressing the ephrin-B2 FL, CTF, or ICD constructs. The ephrin-B2 FL and CTF are detected in the membrane fraction. The ephrin-B2 ICD is detected in the cytosolic and nuclear fractions, indicating translocation of this fragment from the cytosol to the nucleus. Cell treatment with MG132 (0.1 mM, 4 hr) strongly stabilized the ephrin-B2 ICD

Article Snippet: For immunocytochemistry and Western immunoblot analysis the following antibodies were used: C-terminal ephrin-B Ab316 (SAB4300455, Sigma-Aldrich); N-terminal ephrin-B2: (sc-15397, Santa Cruz), N-terminal ephrin-B2 (used in Figure 1 only; MABC127, Millipore); actin (A1978, Sigma-Aldrich); Cell Fractionation Antibody Sampler Kit, containing MEK1/2, AIF, Histone H3, and Vimentin (11843, Cell Signaling Technology); pSrc (Y418) (44660G, Life Technologies); Src (05-184, Millipore); pFAK (Y397) (3283S, Cell signaling technology); Presenilin (C-loop, rb3109, Eurogentec [Prager et al., 2007]); Phosphotyrosine (05-321, Millipore).

Techniques: Binding Assay, Activity Assay, Membrane, Construct, Western Blot, Marker, Fractionation, Control, Cell Fractionation, Translocation Assay